Experimental Resources

Below are a list of microorganisms we work routinely with (as of Jan 2018) and some experimental protocols and recipes we have developed over the years. The latter are coded using an internal coding system (that is irrelevant for wider users). As for the listed microorganisms, we are happy to provide samples to other research groups.

1. Microorganisms

Desulfovibrio vulgaris strain DSM644 (sourced from DSMZ)
Methanococcus maripaludis strain DSM2067 (sourced from DSMZ)
Methanosarcina barkeri strain DSM800 (sourced from DSMZ)
Escherichia coli K-12 strain DSM18039 (sourced from DSMZ)
Shewanella oneidensis strain MR1 (sourced from Rosser group, University of Edinburgh)
Roseobacter sp. AzwK-3b (sourced from Hansel group, Woods Hole Oceanographic Institution, Falmouth, USA)



2. Experimental Protocols (and Culture Media)

OSP01_01:

General protocol for preparing anaerobic media (to be used with any specific media composition)

OSM01_01:

D.vulgaris and M. maripaludis co-culture media (this media can also be used for tri-cultures of D.vulgaris, M. maripaludis, and M. barkeri)

OSP03_01:

Artificial sea water media (used for culturing AzwK-3b and S. oneidensis MR1)

OSP14_00:

General introduction to bio-electrochemical analysis and cell setup